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Chemotaxis: Methods and Protocols

Chemotaxis: Methods and Protocols (Hardback)

Printed to order. Despatched in 2-3 weeks.
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This second edition expands on the first edition with new chapters describing methods for studying

cell movement, molecular components involved in chemotaxis, spatiotemporal

dynamics of signaling components, and quantitative modeling, as well as several

updated chapters from the first edition. Various methods to investigate

directional cell growth and movements are presented in Chapters 1-20. These

chapters contains experimental procedures to visualize and measure migration

behaviors of different kinds of organisms, including chemotropism in the

budding yeast; cell growth and migration of D. discoideum; border cell

migration in Drosophila; chemotaxis of mouse and human neutrophils; and

HIV-induced T cell chemotactic response. Chemotaxis: Methods and Protocols,

Second Edition also contains microscopy procedures for studying breast cancer

cell migration, tumor cell invasion in vivo, and axon guidance. The book concludes

with Chapters 21-27 describing methods that measure spatiotemporal dynamics of

signaling components involved in chemotaxis; introduce imaging techniques, such

as TRIF, BRET, FRET, and single-molecule microscopy; and mathematical models of

experimentally generated chemoattractant gradients. Written in the highly

successful Methods in Molecular Biology series format, chapters include

introductions to their respective topics, lists of the necessary materials and

reagents, step-by-step, readily reproducible laboratory protocols, and tips on

troubleshooting and avoiding known pitfalls.Cutting edge and thorough,

Chemotaxis: Methods and Protocols, Second Edition is a valuable resource for

anyone who is interested in the diverse methodologies that are propelling

chemotaxis research forward.

Science & MathematicsBiology, life sciencesCellular biology or cytology Publisher: Humana Press Inc. Publication Date: 07/06/2016 ISBN-13: 9781493934782  Details: Type: Hardback Format: Books
Availability: Printed to order. Despatched in 2-3 weeks.  

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