A Practical Guide to Molecular Cloning

This new edition of the standard laboratory manual is intended to make molecular cloning faster and easier. It presents techniques tested at the Curie Institute and at other leading laboratories, and lists all commercially available enzymes, vectors, linkers, and other basic products for ready reference. A detailed explanation of protocols is offered, allowing the isolation, cloning, and expression of genes from living species. As well as presenting up-to-date techniques on sequencing, in vitro expression of cloned gene and use of computers for study of nucleic acids, it shows how to isolate DNA-protein complexes and new methods for mutagenesis of cloned genes.